Hotspot

rpoC2

LSC · 16018-20166 · 4149 bp

Back to Corispermum

Pi 0.000603

MarkerSeek score 54.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4149
variable_sites: 5
pi: 0.000603
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCGATTTAAAGTGCGGTCT	AGGAACAGCCCTTCCAATTG	50.0	50.0	58.3	58.1	321 bp	1.0	77.6
rpoC2_p2	AAGTGCGGTCTGTTTTTGGT	AGGAACAGCCCTTCCAATTG	45.0	50.0	58.5	58.1	312 bp	1.0	75.4
rpoC2_p3	AGTGCGGTCTGTTTTTGGTT	AGGAACAGCCCTTCCAATTG	45.0	50.0	58.5	58.1	311 bp	1.0	75.4
rpoC2_p4	AAAGTGCGGTCTGTTTTTGG	AGGAACAGCCCTTCCAATTG	45.0	50.0	57.4	58.1	313 bp	1.0	75.1
rpoC2_p5	CCCGATTTAAAGTGCGGTCT	GCCCTTCCAATTGCGAATTT	50.0	45.0	58.3	57.6	314 bp	1.0	75.0