Hotspot

rpoB

LSC · 23154-26366 · 3213 bp

Back to Corispermum

Pi 0.000778

MarkerSeek score 55.80

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 5
pi: 0.000778
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TATATCCCGCGGCAACAAAA	TGGATAATGTTTGCTACCCGG	45.0	47.6	58.0	58.1	275 bp	1.0	71.7
rpoB_p2	GTTCAGTCGCCGGTTCATAT	TGGATAATGTTTGCTACCCGG	50.0	47.6	58.1	58.1	230 bp	1.0	71.6
rpoB_p3	TCCGCAGCTGCCAATATATC	TGGATAATGTTTGCTACCCGG	50.0	47.6	57.9	58.1	289 bp	1.0	71.2
rpoB_p4	TATATCCCGCGGCAACAAAA	GGATAATGTTTGCTACCCGGA	45.0	47.6	58.0	57.8	274 bp	1.0	70.6
rpoB_p5	GTTCAGTCGCCGGTTCATAT	GGATAATGTTTGCTACCCGGA	50.0	47.6	58.1	57.8	229 bp	1.0	70.6