Hotspot

rpl33

LSC · 67475-67771 · 297 bp

Back to Corispermum

Pi 0.0152

MarkerSeek score 51.80

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 297
variable_sites: 9
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	AACCTTTTGAATTTGATTACGT	TACATAGACGTCCGGTTCGA	27.3	50.0	52.9	58.0	392 bp	1.0	41.8
rpl33_p2	AAACCTTTTGAATTTGATTACGT	TACATAGACGTCCGGTTCGA	26.1	50.0	53.7	58.0	393 bp	1.0	41.8
rpl33_p3	AAAACCTTTTGAATTTGATTACGT	TACATAGACGTCCGGTTCGA	25.0	50.0	54.5	58.0	394 bp	1.0	41.8
rpl33_p4	ACCTTTTGAATTTGATTACGTAG	TACATAGACGTCCGGTTCGA	30.4	50.0	53.4	58.0	391 bp	1.0	41.8
rpl33_p5	AACCTTTTGAATTTGATTACGT	TGTCCCCCTACTTGAAAGGA	27.3	50.0	52.9	57.6	415 bp	1.0	41.7