Hotspot

ndhD

SSC · 113428-115041 · 1614 bp

Back to Corispermum

Pi 0.0033

MarkerSeek score 55.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1614
variable_sites: 9
pi: 0.0033
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCGTCAGTCTTGCTTCTTCT	AAGAGATGTGAATCCGCCTG	50.0	50.0	58.2	57.7	1799 bp	1.0	76.1
ndhD_p2	AATGACTAACTCCGCGTCAG	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.7	57.7	1812 bp	1.0	75.6
ndhD_p3	GCGTCAGTCTTGCTTCTTCT	GAGATGTGAATCCGCCTGTC	50.0	55.0	58.2	58.4	1797 bp	1.0	75.3
ndhD_p4	AATGACTAACTCCGCGTCAG	GAGATGTGAATCCGCCTGTC	50.0	55.0	57.7	58.4	1810 bp	1.0	74.8
ndhD_p5	CTAACTCCGCGTCAGTCTTG	AAGAGATGTGAATCCGCCTG	55.0	50.0	58.4	57.7	1807 bp	1.0	74.8