Hotspot

rpl22-rps19

LSC · 84050-84076 · 27 bp

Back to Corispermum

Pi 0.0741

MarkerSeek score 67.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 27
variable_sites: 4
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CACTGACGCCACTCAAACAT	AGGAGAATTCGCACCAACTC	50.0	50.0	58.8	57.9	210 bp	1.0	74.6
rpl22-rps19_p2	CACTGACGCCACTCAAACAT	GGAGAATTCGCACCAACTCT	50.0	50.0	58.8	57.9	209 bp	1.0	74.6
rpl22-rps19_p3	ACTGACGCCACTCAAACATA	AGGAGAATTCGCACCAACTC	45.0	50.0	57.2	57.9	209 bp	1.0	74.1
rpl22-rps19_p4	ACTGACGCCACTCAAACATA	GGAGAATTCGCACCAACTCT	45.0	50.0	57.2	57.9	208 bp	1.0	74.1
rpl22-rps19_p5	CACTGACGCCACTCAAACAT	ATTTCCGAGGACACGCAAAA	50.0	45.0	58.8	58.4	187 bp	1.0	72.3