Hotspot

petA-psbJ

LSC · 63010-63981 · 972 bp

Back to Corispermum

Pi 0.00154

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 3
pi: 0.00154
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCGCGTACAAGGTCTTTT	ATTCCGGATTAGGCTCATCG	47.4	50.0	58.0	57.2	1111 bp	1.0	66.2
petA-psbJ_p2	CGCGTACAAGGTCTTTTGTT	ATTCCGGATTAGGCTCATCG	45.0	50.0	57.0	57.2	1109 bp	1.0	66.0
petA-psbJ_p3	CGCGTACAAGGTCTTTTGTTC	ATTCCGGATTAGGCTCATCG	47.6	50.0	58.1	57.2	1109 bp	1.0	65.4
petA-psbJ_p4	CTGCGCGTACAAGGTCTTTT	ATTCCGGATTAGGCTCATCG	50.0	50.0	59.1	57.2	1112 bp	1.0	65.1
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTAGGCTCATCG	50.0	50.0	56.8	57.2	1032 bp	1.0	64.9