Hotspot

psaA-ycf3

LSC · 41454-42161 · 708 bp

Back to Corispermum

Pi 0.00377

MarkerSeek score 54.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 4
pi: 0.00377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	887-905 bp	1.0	80.9
psaA-ycf3_p2	TGAAAAATGACCGGGTCTGG	CAGAGGCTTGGTTCGATCAA	50.0	50.0	58.1	57.9	902-920 bp	1.0	80.4
psaA-ycf3_p3	TGAAAAATGACCGGGTCTGG	TGAGGCGCATAATTGGTTGA	50.0	45.0	58.1	57.9	832-850 bp	1.0	80.3
psaA-ycf3_p4	TGAAAAATGACCGGGTCTGG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	821-839 bp	1.0	80.3
psaA-ycf3_p5	TGAAAAATGACCGGGTCTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	889-907 bp	1.0	80.0