Hotspot

atpH-atpI

LSC · 13408-14108 · 701 bp

Back to Corispermum

Pi 0.00309

MarkerSeek score 54.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 4
pi: 0.00309
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	786-796 bp	1.0	79.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	756-766 bp	1.0	78.9
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	842-852 bp	1.0	78.6
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	802-812 bp	1.0	78.3
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	812-822 bp	1.0	78.3