Hotspot

trnH-GUG-psbA

LSC · 99-458 · 360 bp

Back to Corispermum

Pi 0.013

MarkerSeek score 63.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 9
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	429-430 bp	1.0	80.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	443-444 bp	1.0	80.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	442-443 bp	1.0	80.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	444-445 bp	1.0	80.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	478-479 bp	1.0	78.0