Hotspot

ycf3

Genome · 85395-87387 · 1993 bp

Back to Corethrodendron

Pi 0

MarkerSeek score 53.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: Genome
length_bp: 1993
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AACAAAATAATAAACAGAGTGTTC	GGGGATTATTTTCTCACGCG	25.0	50.0	52.3	57.0	2070-2071 bp	1.0	40.0
ycf3_p2	AACAAAATAATAAACAGAGTGTTC	TGGGGGATTATTTTCTCACGC	25.0	47.6	52.3	58.3	2072-2073 bp	1.0	40.0
ycf3_p3	AACAAAATAATAAACAGAGTGTTC	GGGGATTATTTTCTCACGCGA	25.0	47.6	52.3	58.4	2070-2071 bp	1.0	40.0
ycf3_p4	AACAAAATAATAAACAGAGTGTTC	GGGGGATTATTTTCTCACGC	25.0	50.0	52.3	56.6	2071-2072 bp	1.0	40.0
ycf3_p5	AACAAAATAATAAACAGAGTGTTC	TTGTACAATTCCTTGGGGGA	25.0	45.0	52.3	56.3	2085-2086 bp	1.0	40.0