Hotspot

rpl32-trnL-UAG

Genome · 107069-107654 · 586 bp

Back to Corethrodendron

Pi 0.00341

MarkerSeek score 60.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 586
variable_sites: 2
pi: 0.00341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGACAGCATTGAAAGCCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	703 bp	1.0	79.2
rpl32-trnL-UAG_p2	TTGGACAGCATTGAAAGCCT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	704 bp	1.0	79.2
rpl32-trnL-UAG_p3	TGGACAGCATTGAAAGCCTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	696 bp	1.0	78.7
rpl32-trnL-UAG_p4	TTGGACAGCATTGAAAGCCT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	697 bp	1.0	78.7
rpl32-trnL-UAG_p5	TGGACAGCATTGAAAGCCTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.0	57.8	701 bp	1.0	78.7