Hotspot

atpB-rbcL

Genome · 96549-97289 · 741 bp

Back to Corethrodendron

Pi 0

MarkerSeek score 58.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 741
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGCGATACGTCCCAGATTTT	AACACCAGCTTTGAACCCAA	50.0	45.0	58.1	58.1	863-864 bp	1.0	78.6
atpB-rbcL_p2	GGCGATACGTCCCAGATTTT	AGTTTCTGTTTGCGGTGACA	50.0	45.0	58.1	58.3	833-834 bp	1.0	77.7
atpB-rbcL_p3	GGCGATACGTCCCAGATTTT	GCTTTGAACCCAACACTTGC	50.0	50.0	58.1	58.4	856-857 bp	1.0	76.3
atpB-rbcL_p4	GGCGATACGTCCCAGATTTT	CCAGCTTTGAACCCAACACT	50.0	50.0	58.1	58.6	859-860 bp	1.0	74.9
atpB-rbcL_p5	GGCGATACGTCCCAGATTTT	TAACACCAGCTTTGAACCCA	50.0	45.0	58.1	57.3	864-865 bp	1.0	73.8