Hotspot

ndhC-trnM-CAU

Genome · 93243-94461 · 1219 bp

Back to Corethrodendron

Pi 0.00082
MarkerSeek score 59.20
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
Genome
length_bp
1219
variable_sites
1
pi
0.00082
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 CTGGCCCTTTGTTAATCGGA TTGAACCAATGACTCCTGCC 50.0 50.0 57.9 58.1 1392 bp 1.0 78.4
ndhC-trnM-CAU_p2 TCTGGCCCTTTGTTAATCGG TTGAACCAATGACTCCTGCC 50.0 50.0 57.9 58.1 1393 bp 1.0 78.4
ndhC-trnM-CAU_p3 TGGCCCTTTGTTAATCGGAG TTGAACCAATGACTCCTGCC 50.0 50.0 57.9 58.1 1391 bp 1.0 78.4
ndhC-trnM-CAU_p4 GCCCTTTGTTAATCGGAGCT TTGAACCAATGACTCCTGCC 50.0 50.0 58.2 58.1 1389 bp 1.0 77.3
ndhC-trnM-CAU_p5 TGGCCCTTTGTTAATCGGAG CTCCTGCCGTATGAAAGCAA 50.0 50.0 57.9 58.3 1379 bp 1.0 77.0