Hotspot

psaA-ycf3

Genome · 84487-85394 · 908 bp

Back to Corethrodendron

Pi 0.00332

MarkerSeek score 61.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 908
variable_sites: 3
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCCCATTCCTCGAAAGAAG	ATAGCGCTTACCCCTGGTAA	50.0	50.0	57.9	58.2	1075-1079 bp	1.0	78.0
psaA-ycf3_p2	TGCCCATTCCTCGAAAGAAG	TAGCGCTTACCCCTGGTAAT	50.0	50.0	57.9	58.2	1074-1078 bp	1.0	78.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATAGCGCTTACCCCTGGTAA	50.0	50.0	57.9	58.2	1074-1078 bp	1.0	78.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACCCCTGGTAAT	50.0	50.0	57.9	58.2	1073-1077 bp	1.0	78.0
psaA-ycf3_p5	TGCCCATTCCTCGAAAGAAG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	1111-1115 bp	1.0	77.4