Hotspot

trnV-GAC-rps12

Genome · 8868-10590 · 1723 bp

Back to Corethrodendron

Pi 0.00058
MarkerSeek score 58.60
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
Genome
length_bp
1723
variable_sites
1
pi
0.00058
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1835 bp 1.0 78.1
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1839 bp 1.0 78.1
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1834 bp 1.0 78.1
trnV-GAC-rps12_p4 GATGACTTCCACCACGTCAA GGTTAAGCGTACTCTGGCAA 50.0 50.0 57.8 57.9 1833 bp 1.0 78.0
trnV-GAC-rps12_p5 ATGACTTCCACCACGTCAAG GGTTAAGCGTACTCTGGCAA 50.0 50.0 57.8 57.9 1832 bp 1.0 78.0