primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC_p1	trnA-UGC	1	AGCATAGATCAAACTGCCGA	TGATTTACTTCACGGGCGAG	20	20	45.0	50.0	57.0	58.0	0.0	0.0	0.992593	4882	5759	1075-1075	1.000000	72.1
trnA-UGC_p2	trnA-UGC	2	AGCATAGATCAAACTGCCGA	GACTACTTCCTGCATGCTCC	20	20	45.0	55.0	57.0	58.1	0.0	20.3	1.041265	4882	5759	994-994	1.000000	71.7
trnA-UGC_p3	trnA-UGC	3	AGCATAGATCAAACTGCCGA	AGGTCTCTGGTTCAAGTCCA	20	20	45.0	50.0	57.0	57.9	0.0	0.0	1.091164	4882	5759	1057-1057	1.000000	71.3
trnA-UGC_p4	trnA-UGC	4	AGCATAGATCAAACTGCCGA	TACTTCCTGCATGCTCCAAC	20	20	45.0	50.0	57.0	57.9	0.0	20.3	1.101871	4882	5759	991-991	1.000000	71.2
trnA-UGC_p5	trnA-UGC	5	CGAATCGGAAAAATGGAGTGC	TGATTTACTTCACGGGCGAG	21	20	47.6	50.0	58.2	58.0	0.0	0.0	1.218904	4882	5759	1058-1058	1.000000	70.2
trnI_p1	trnI	1	GGAGCATGCAGGAAGTAGTC	GGTAAAGAGAGGGATGGGGT	20	20	55.0	55.0	58.1	58.1	22.0	0.0	0.158556	5824	6855	1152-1152	1.000000	78.8
trnI_p2	trnI	2	GGAGCATGCAGGAAGTAGTC	AGAGAGGGATGGGGTTTCTC	20	20	55.0	55.0	58.1	58.1	22.0	0.0	0.166833	5824	6855	1147-1147	1.000000	78.7
trnI_p3	trnI	3	GGAGCATGCAGGAAGTAGTC	AGAGGGATGGGGTTTCTCTC	20	20	55.0	55.0	58.1	58.1	22.0	0.0	0.166833	5824	6855	1145-1145	1.000000	78.7
trnI_p4	trnI	4	GGAGCATGCAGGAAGTAGTC	GAGAGGGATGGGGTTTCTCT	20	20	55.0	55.0	58.1	58.1	22.0	0.0	0.166833	5824	6855	1146-1146	1.000000	78.7
trnI_p5	trnI	5	GGAGCATGCAGGAAGTAGTC	GATGGGGTTTCTCTCGCTTT	20	20	55.0	50.0	58.1	57.9	22.0	0.0	0.168598	5824	6855	1140-1140	1.000000	78.7
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GATGACTTCCACCACGTCAA	GAGGTTAAGCGTACTCTGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.239919	8868	10590	1835-1835	1.000000	78.1
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	AACTGATGACTTCCACCACG	GAGGTTAAGCGTACTCTGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.245933	8868	10590	1839-1839	1.000000	78.1
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	ATGACTTCCACCACGTCAAG	GAGGTTAAGCGTACTCTGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.245933	8868	10590	1834-1834	1.000000	78.1
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	GATGACTTCCACCACGTCAA	GGTTAAGCGTACTCTGGCAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.252768	8868	10590	1833-1833	1.000000	78.0
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	ATGACTTCCACCACGTCAAG	GGTTAAGCGTACTCTGGCAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.258782	8868	10590	1832-1832	1.000000	78.0
rpl14-rps8_p1	rpl14-rps8	1	GCCCGAGAATTGAGACAGTT	TCACGATGCTTTCTGACGTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.249520	26924	27548	794-795	1.000000	78.2
rpl14-rps8_p2	rpl14-rps8	2	GCCCGAGAATTGAGACAGTT	TTCACGATGCTTTCTGACGT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.249520	26924	27548	795-796	1.000000	78.2
rpl14-rps8_p3	rpl14-rps8	3	CGCAATAGCCCGAGAATTGA	TCACGATGCTTTCTGACGTT	20	20	50.0	45.0	58.4	57.9	0.0	0.0	0.566982	26924	27548	801-802	1.000000	75.7
rpl14-rps8_p4	rpl14-rps8	4	CGCAATAGCCCGAGAATTGA	TTCACGATGCTTTCTGACGT	20	20	50.0	45.0	58.4	57.9	0.0	0.0	0.566982	26924	27548	802-803	1.000000	75.7
rpl14-rps8_p5	rpl14-rps8	5	AGCCCGAGAATTGAGACAGT	TCACGATGCTTTCTGACGTT	20	20	50.0	45.0	58.7	57.9	0.0	0.0	0.878009	26924	27548	795-796	1.000000	73.2
psbE_p1	psbE	1	GAACAGCCAACCATCGTACT	GGAATCGATCCCCCTTTGAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.126058	37663	37914	358-358	1.000000	79.0
psbE_p2	psbE	2	AACAGCCAACCATCGTACTG	GGAATCGATCCCCCTTTGAC	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.222885	37663	37914	357-357	1.000000	78.2
psbE_p3	psbE	3	GTGAACAGCCAACCATCGTA	GGAATCGATCCCCCTTTGAC	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.227962	37663	37914	360-360	1.000000	78.2
psbE_p4	psbE	4	TGAACAGCCAACCATCGTAC	GGAATCGATCCCCCTTTGAC	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.227962	37663	37914	359-359	1.000000	78.2
psbE_p5	psbE	5	GAACAGCCAACCATCGTACT	TCGATCCCCCTTTGACTGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.336043	37663	37914	354-354	1.000000	77.3
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	ACTGATAAAAAGACCTGCGGT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.436023	37915	38836	1109-1109	1.000000	68.6
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	ACTGATAAAAAGACCTGCGGT	20	21	50.0	42.9	57.9	57.6	6.4	0.0	1.470018	37915	38836	1098-1098	1.000000	68.3
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	ACTGATAAAAAGACCTGCGGT	20	21	50.0	42.9	57.8	57.6	24.9	0.0	1.575027	37915	38836	1021-1021	1.000000	67.5
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	ACTGATAAAAAGACCTGCGGT	20	21	55.0	42.9	58.2	57.6	0.9	0.0	1.616615	37915	38836	1016-1016	1.000000	67.1
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	ACTGATAAAAAGACCTGCGGT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.655976	37915	38836	1100-1100	1.000000	66.8
rpl20_p1	rpl20	1	CTACCACCTTCCCGGAGTTA	AGGAAGGATAAAAGAATAACTGA	20	23	55.0	30.4	58.1	52.8	0.7	0.0	8.313435	41859	42221	465-465	1.000000	40.2
rpl20_p2	rpl20	2	CTACCACCTTCCCGGAGTTA	AGGAAGGATAAAAGAATAACTGAAAGA	20	27	55.0	29.6	58.1	56.7	0.7	0.0	8.399851	41859	42221	465-465	1.000000	40.2
rpl20_p3	rpl20	3	CTACCACCTTCCCGGAGTTA	ACTAGGAAGGATAAAAGAATAACTGA	20	26	55.0	30.8	58.1	55.7	0.7	0.0	8.472234	41859	42221	468-468	1.000000	40.2
rpl20_p4	rpl20	4	CTACCACCTTCCCGGAGTTA	GACTAGGAAGGATAAAAGAATAACTGA	20	27	55.0	33.3	58.1	56.7	0.7	0.0	8.473771	41859	42221	469-469	1.000000	40.2
rpl20_p5	rpl20	5	CTACCACCTTCCCGGAGTTA	AGACTAGGAAGGATAAAAGAATAACTG	20	27	55.0	33.3	58.1	56.7	0.7	0.0	8.480659	41859	42221	470-470	1.000000	40.2
atpF_p1	atpF	1	TGCACGAATGGTTACCATGA	CCATAAGAGGAGATGCGATCA	20	21	45.0	47.6	57.5	57.1	18.5	0.0	2.431131	54104	55367	1401-1401	1.000000	60.6
atpF_p2	atpF	2	TGCACGAATGGTTACCATGA	TCCATAAGAGGAGATGCGATC	20	21	45.0	47.6	57.5	56.8	18.5	0.0	2.708776	54104	55367	1402-1402	1.000000	58.4
atpF_p3	atpF	3	ATCTGCACGAATGGTTACCA	CCATAAGAGGAGATGCGATCA	20	21	45.0	47.6	57.2	57.1	6.5	0.0	2.729597	54104	55367	1404-1404	1.000000	58.2
atpF_p4	atpF	4	CTGCACGAATGGTTACCATG	CCATAAGAGGAGATGCGATCA	20	21	50.0	47.6	57.2	57.1	18.5	0.0	2.780614	54104	55367	1402-1402	1.000000	57.8
atpF_p5	atpF	5	TGCACGAATGGTTACCATGA	CCATAAGAGGAGATGCGATCAT	20	22	45.0	45.5	57.5	57.5	18.5	0.0	2.950054	54104	55367	1401-1401	1.000000	56.5
rps2_p1	rps2	1	TTGCACAATTTTGTAATGGT	AAAGAGAAGGTTCCGTTGGA	20	20	30.0	45.0	52.1	56.7	0.0	0.0	7.236967	57893	58603	906-907	1.000000	40.1
rps2_p2	rps2	2	TTGCACAATTTTGTAATGGT	AAGAGAAGGTTCCGTTGGAA	20	20	30.0	45.0	52.1	56.7	0.0	0.0	7.236967	57893	58603	905-906	1.000000	40.1
rps2_p3	rps2	3	TTTGCACAATTTTGTAATGGT	AAAGAGAAGGTTCCGTTGGA	21	20	28.6	45.0	53.0	56.7	0.0	0.0	7.279026	57893	58603	907-908	1.000000	40.1
rps2_p4	rps2	4	TTTGCACAATTTTGTAATGGT	AAGAGAAGGTTCCGTTGGAA	21	20	28.6	45.0	53.0	56.7	0.0	0.0	7.279026	57893	58603	906-907	1.000000	40.1
rps2_p5	rps2	5	TCTATGAATTTCCCACCAAAA	AAGAGAAGGTTCCGTTGGAA	21	20	33.3	45.0	53.0	56.7	0.0	0.0	7.350368	57893	58603	865-866	1.000000	40.1
rps2-rpoC2_p1	rps2-rpoC2	1	GTGCCATTCGAGGATTCCAT	CCGGTCAAGGCAACATAACA	20	20	50.0	50.0	58.0	58.5	5.3	0.0	0.497902	58604	58882	493-494	1.000000	76.3
rps2-rpoC2_p2	rps2-rpoC2	2	AAGGTGCCATTCGAGGATTC	CCGGTCAAGGCAACATAACA	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.514897	58604	58882	496-497	1.000000	76.2
rps2-rpoC2_p3	rps2-rpoC2	3	TGCCATTCGAGGATTCCATT	CCGGTCAAGGCAACATAACA	20	20	45.0	50.0	57.3	58.5	5.3	0.0	1.205530	58604	58882	492-493	1.000000	70.7
rps2-rpoC2_p4	rps2-rpoC2	4	GGCCAAAATGAACTCCTGCT	CCGGTCAAGGCAACATAACA	20	20	50.0	50.0	58.7	58.5	0.0	0.0	1.210230	58604	58882	460-461	1.000000	70.7
rps2-rpoC2_p5	rps2-rpoC2	5	GTGCCATTCGAGGATTCCAT	CCCGGTCAAGGCAACATAAC	20	20	50.0	55.0	58.0	59.2	5.3	0.0	1.218373	58604	58882	494-495	1.000000	70.6
psaA-ycf3_p1	psaA-ycf3	1	TGCCCATTCCTCGAAAGAAG	ATAGCGCTTACCCCTGGTAA	20	20	50.0	50.0	57.9	58.2	4.1	18.7	0.316540	84487	85394	1075-1079	1.000000	78.0
psaA-ycf3_p2	psaA-ycf3	2	TGCCCATTCCTCGAAAGAAG	TAGCGCTTACCCCTGGTAAT	20	20	50.0	50.0	57.9	58.2	4.1	18.7	0.316540	84487	85394	1074-1078	1.000000	78.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	ATAGCGCTTACCCCTGGTAA	20	20	50.0	50.0	57.9	58.2	4.1	18.7	0.318022	84487	85394	1074-1078	1.000000	78.0
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACCCCTGGTAAT	20	20	50.0	50.0	57.9	58.2	4.1	18.7	0.318022	84487	85394	1073-1077	1.000000	78.0
psaA-ycf3_p5	psaA-ycf3	5	TGCCCATTCCTCGAAAGAAG	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.9	57.7	4.1	19.9	0.388994	84487	85394	1111-1115	1.000000	77.4
ycf3_p1	ycf3	1	AACAAAATAATAAACAGAGTGTTC	GGGGATTATTTTCTCACGCG	24	20	25.0	50.0	52.3	57.0	0.0	3.5	10.760666	85395	87387	2070-2071	1.000000	40.0
ycf3_p2	ycf3	2	AACAAAATAATAAACAGAGTGTTC	TGGGGGATTATTTTCTCACGC	24	21	25.0	47.6	52.3	58.3	0.0	0.0	11.071609	85395	87387	2072-2073	1.000000	40.0
ycf3_p3	ycf3	3	AACAAAATAATAAACAGAGTGTTC	GGGGATTATTTTCTCACGCGA	24	21	25.0	47.6	52.3	58.4	0.0	15.9	11.164614	85395	87387	2070-2071	1.000000	40.0
ycf3_p4	ycf3	4	AACAAAATAATAAACAGAGTGTTC	GGGGGATTATTTTCTCACGC	24	20	25.0	50.0	52.3	56.6	0.0	0.0	11.166006	85395	87387	2071-2072	1.000000	40.0
ycf3_p5	ycf3	5	AACAAAATAATAAACAGAGTGTTC	TTGTACAATTCCTTGGGGGA	24	20	25.0	45.0	52.3	56.3	0.0	3.6	11.404296	85395	87387	2085-2086	1.000000	40.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	88914	90064	1201-1201	1.000000	79.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	88914	90064	1232-1232	1.000000	78.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	88914	90064	1209-1209	1.000000	78.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	88914	90064	1240-1240	1.000000	78.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CCCATTGTGAATTCCAGGGT	20	20	55.0	50.0	57.9	57.8	0.0	2.3	0.369229	88914	90064	1294-1294	1.000000	77.1
ndhC-trnM-CAU_p1	ndhC-trnM-CAU	1	CTGGCCCTTTGTTAATCGGA	TTGAACCAATGACTCCTGCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.213084	93243	94461	1392-1392	1.000000	78.4
ndhC-trnM-CAU_p2	ndhC-trnM-CAU	2	TCTGGCCCTTTGTTAATCGG	TTGAACCAATGACTCCTGCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.213084	93243	94461	1393-1393	1.000000	78.4
ndhC-trnM-CAU_p3	ndhC-trnM-CAU	3	TGGCCCTTTGTTAATCGGAG	TTGAACCAATGACTCCTGCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.213084	93243	94461	1391-1391	1.000000	78.4
ndhC-trnM-CAU_p4	ndhC-trnM-CAU	4	GCCCTTTGTTAATCGGAGCT	TTGAACCAATGACTCCTGCC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.338863	93243	94461	1389-1389	1.000000	77.3
ndhC-trnM-CAU_p5	ndhC-trnM-CAU	5	TGGCCCTTTGTTAATCGGAG	CTCCTGCCGTATGAAAGCAA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.384945	93243	94461	1379-1379	1.000000	77.0
atpB-rbcL_p1	atpB-rbcL	1	GGCGATACGTCCCAGATTTT	AACACCAGCTTTGAACCCAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.191233	96549	97289	863-864	1.000000	78.6
atpB-rbcL_p2	atpB-rbcL	2	GGCGATACGTCCCAGATTTT	AGTTTCTGTTTGCGGTGACA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.305303	96549	97289	833-834	1.000000	77.7
atpB-rbcL_p3	atpB-rbcL	3	GGCGATACGTCCCAGATTTT	GCTTTGAACCCAACACTTGC	20	20	50.0	50.0	58.1	58.4	0.0	0.0	0.479722	96549	97289	856-857	1.000000	76.3
atpB-rbcL_p4	atpB-rbcL	4	GGCGATACGTCCCAGATTTT	CCAGCTTTGAACCCAACACT	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.647748	96549	97289	859-860	1.000000	74.9
atpB-rbcL_p5	atpB-rbcL	5	GGCGATACGTCCCAGATTTT	TAACACCAGCTTTGAACCCA	20	20	50.0	45.0	58.1	57.3	0.0	0.0	0.789121	96549	97289	864-865	1.000000	73.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGACAGCATTGAAAGCCTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.124629	107069	107654	703-703	1.000000	79.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTGGACAGCATTGAAAGCCT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.124629	107069	107654	704-704	1.000000	79.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGGACAGCATTGAAAGCCTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.185356	107069	107654	696-696	1.000000	78.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTGGACAGCATTGAAAGCCT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.185356	107069	107654	697-697	1.000000	78.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGGACAGCATTGAAAGCCTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.0	57.8	0.0	0.0	0.196647	107069	107654	701-701	1.000000	78.7