Hotspot

ndhE-ndhG

SSC · 116744-116956 · 213 bp

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Pi 0.0156
MarkerSeek score 62.50
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
213
variable_sites
5
pi
0.0156
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 GACACATAAGGGCTCTAACCA AGGTGCAATTGCTGTAGCTC 47.6 50.0 57.1 58.5 341 bp 1.0 61.6
ndhE-ndhG_p2 AGTTCAAGACACATAAGGGCTC AGGTGCAATTGCTGTAGCTC 45.5 50.0 58.1 58.5 348 bp 1.0 60.4
ndhE-ndhG_p3 GTTCAAGACACATAAGGGCTCT AGGTGCAATTGCTGTAGCTC 45.5 50.0 58.1 58.5 347 bp 1.0 60.4
ndhE-ndhG_p4 AGTTCAAGACACATAAGGGCT AGGTGCAATTGCTGTAGCTC 42.9 50.0 56.9 58.5 348 bp 1.0 60.2
ndhE-ndhG_p5 TCAAGACACATAAGGGCTCT AGGTGCAATTGCTGTAGCTC 45.0 50.0 55.9 58.5 345 bp 1.0 59.6