Hotspot

ndhF-rpl32

SSC · 111298-112033 · 736 bp

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Pi 0.00543
MarkerSeek score 62.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
736
variable_sites
6
pi
0.00543
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAAAAAGAAG AACGCTGTCCAATATCCCTT 42.9 45.0 57.3 56.9 920-926 bp 1.0 58.4
ndhF-rpl32_p2 TCGGAAAAAGAAGAAGTCCCA AACGCTGTCCAATATCCCTT 42.9 45.0 57.2 56.9 912-918 bp 1.0 57.1
ndhF-rpl32_p3 TGTTGCTGTCGGAAAAAGAA AACGCTGTCCAATATCCCTT 40.0 45.0 56.1 56.9 920-926 bp 1.0 56.7
ndhF-rpl32_p4 TTGTTGCTGTCGGAAAAAGA AACGCTGTCCAATATCCCTT 40.0 45.0 56.1 56.9 921-927 bp 1.0 56.7
ndhF-rpl32_p5 CGGAAAAAGAAGAAGTCCCAC AACGCTGTCCAATATCCCTT 47.6 45.0 57.1 56.9 911-917 bp 1.0 56.7