Hotspot

rps12-psbB

LSC · 70975-72193 · 1219 bp

Back to Cordia

Pi 0.00384
MarkerSeek score 61.50
Primer available yes
Label rps12-psbB

Feature Metadata

label_name
rps12-psbB
feature_type
igs
region
LSC
length_bp
1219
variable_sites
7
pi
0.00384
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-psbB_p1 CTAGTACATGTTCCTCGGCG CGATACCAAGGCAAACCCAT 55.0 50.0 57.9 58.2 1346-1358 bp 1.0 77.8
rps12-psbB_p2 CTAGTACATGTTCCTCGGCG ACACGATACCAAGGCAAACC 55.0 50.0 57.9 58.5 1349-1361 bp 1.0 75.9
rps12-psbB_p3 CTAGTACATGTTCCTCGGCG TGAACACGATACCAAGGCAA 55.0 45.0 57.9 57.4 1352-1364 bp 1.0 75.3
rps12-psbB_p4 CTAGTACATGTTCCTCGGCG GCAACCAGAGCTGTATGCAT 55.0 50.0 57.9 58.6 1418-1430 bp 1.0 74.7
rps12-psbB_p5 CCTAGTACATGTTCCTCGGC CGATACCAAGGCAAACCCAT 55.0 50.0 57.5 58.2 1347-1359 bp 1.0 74.7