Hotspot

rpl20-rps12

LSC · 70063-70860 · 798 bp

Back to Cordia

Pi 0.00544
MarkerSeek score 62.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
798
variable_sites
6
pi
0.00544
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 GAGCCCCTCGAAAGTTTGAT CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 906-909 bp 1.0 78.9
rpl20-rps12_p2 AGCCCCTCGAAAGTTTGATG CGCCGAGGAACATGTACTAG 50.0 55.0 58.2 57.9 905-908 bp 1.0 78.4
rpl20-rps12_p3 ATGAGCCCCTCGAAAGTTTG CGCCGAGGAACATGTACTAG 50.0 55.0 58.2 57.9 908-911 bp 1.0 78.4
rpl20-rps12_p4 TCTCCGAGCTACATATCCCC CGCCGAGGAACATGTACTAG 55.0 55.0 57.8 57.9 860-863 bp 1.0 78.1
rpl20-rps12_p5 TTTGTTCTACGTCTCCGAGC CGCCGAGGAACATGTACTAG 50.0 55.0 57.7 57.9 871-874 bp 1.0 77.1