Hotspot

psbE-petL

LSC · 65730-66950 · 1221 bp

Back to Cordia

Pi 0.00821
MarkerSeek score 64.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1221
variable_sites
15
pi
0.00821
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCA 50.0 45.0 57.9 56.9 1364-1368 bp 1.0 71.6
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGCCGCTAGTAGAAAACCAA 50.0 45.0 57.9 56.9 1363-1367 bp 1.0 71.6
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC AAGCCGCTAGTAGAAAACCA 50.0 45.0 57.8 56.9 1287-1291 bp 1.0 70.8
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AGCCGCTAGTAGAAAACCAA 50.0 45.0 57.8 56.9 1286-1290 bp 1.0 70.8
psbE-petL_p5 ACGAATAACCAACCCGCAAT AGCCGCTAGTAGAAAACCAA 45.0 45.0 58.2 56.9 1372-1376 bp 1.0 70.6