Hotspot

petA-psbJ

LSC · 64120-64952 · 833 bp

Back to Cordia

Pi 0.012
MarkerSeek score 69.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
833
variable_sites
13
pi
0.012
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC GCCGATACTACTGGAAGGAT 50.0 50.0 57.8 56.2 851-1111 bp 1.0 69.4
petA-psbJ_p2 CCATTACGTGTTCAAGGCCT GCCGATACTACTGGAAGGAT 50.0 50.0 58.2 56.2 866-1126 bp 1.0 69.2
petA-psbJ_p3 TCCATTACGTGTTCAAGGCC GCCGATACTACTGGAAGGAT 50.0 50.0 58.2 56.2 867-1127 bp 1.0 69.2
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC GCCGATACTACTGGAAGGATT 50.0 47.6 57.8 57.0 851-1111 bp 1.0 67.5
petA-psbJ_p5 CCATTACGTGTTCAAGGCCT GCCGATACTACTGGAAGGATT 50.0 47.6 58.2 57.0 866-1126 bp 1.0 67.3