Hotspot

ndhC-trnV-UAC

LSC · 51331-52467 · 1137 bp

Back to Cordia

Pi 0.00704
MarkerSeek score 56.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1137
variable_sites
12
pi
0.00704
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT TGCATGTTGGGTCTGTGAAA 50.0 45.0 57.9 57.9 1333-1335 bp 1.0 79.2
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AAAGTGCATGTTGGGTCTGT 50.0 45.0 57.9 57.9 1337-1339 bp 1.0 79.1
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1268-1270 bp 1.0 78.2
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GCATGTTGGGTCTGTGAAAC 50.0 50.0 57.9 57.6 1332-1334 bp 1.0 76.3
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1267-1269 bp 1.0 75.4