Hotspot

psaA-ycf3

LSC · 42722-43363 · 642 bp

Back to Cordia

Pi 0.00521
MarkerSeek score 62.70
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
642
variable_sites
5
pi
0.00521
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 817-820 bp 1.0 79.6
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 799-802 bp 1.0 79.6
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT CGATCAAGCCGCTGAGTATT 50.0 50.0 57.9 58.1 819-822 bp 1.0 78.8
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT AGCCGCTGAGTATTGGAAAC 50.0 50.0 57.9 58.3 813-816 bp 1.0 77.9
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT CAAGCCGCTGAGTATTGGAA 50.0 50.0 57.9 58.3 815-818 bp 1.0 77.9