Hotspot

trnG-GCC-trnfM-CAU

LSC · 37439-37585 · 147 bp

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Pi 0.0181
MarkerSeek score 62.30
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
147
variable_sites
4
pi
0.0181
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 249-250 bp 1.0 80.6
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 250-251 bp 1.0 80.6
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC TTCAGTTCTTCTCTTCGGCG 50.0 50.0 58.1 57.9 271-272 bp 1.0 80.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT TTCAGTTCTTCTCTTCGGCG 50.0 50.0 58.1 57.9 272-273 bp 1.0 80.2
trnG-GCC-trnfM-CAU_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 290-291 bp 1.0 80.1