Hotspot

ycf2-trnI-CAU

IRa · 158512-158654 · 143 bp

Back to Corchorus

Pi 0.156

MarkerSeek score 75.00

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 143
variable_sites: 19
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GTTGAACCCTGTGAATTGCG	GGTCCCACTCTTCTACCCTT	50.0	55.0	57.9	58.0	1020-1111 bp	1.0	89.6
ycf2-trnI-CAU_p2	CCAGCGACGGTTTCATTAGA	GGTCCCACTCTTCTACCCTT	50.0	55.0	58.0	58.0	1410-1501 bp	1.0	89.1
ycf2-trnI-CAU_p3	TCCAGCGACGGTTTCATTAG	GGTCCCACTCTTCTACCCTT	50.0	55.0	58.0	58.0	1411-1502 bp	1.0	89.1
ycf2-trnI-CAU_p4	CCAGCGACGGTTTCATTAGA	GAAGAGTTCGACCCAATGCT	50.0	50.0	58.0	57.9	1345-1436 bp	1.0	89.0
ycf2-trnI-CAU_p5	AATCATGGTCCTTGCGGATC	GGTCCCACTCTTCTACCCTT	50.0	55.0	58.0	58.0	1502-1593 bp	1.0	88.7