Hotspot

rpl23-trnI-CAU

IRb · 90494-90975 · 482 bp

Back to Corchorus

Pi 0.133
MarkerSeek score 80.90
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
IRb
length_bp
482
variable_sites
52
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 ACCCAATGCTTTATCTCTGTCC TGGCGAATTCGTAGGTTCAA 45.5 45.0 58.1 57.5 566-633 bp 1.0 70.4
rpl23-trnI-CAU_p2 ACCCAATGCTTTATCTCTGTCC TTGGCGAATTCGTAGGTTCA 45.5 45.0 58.1 57.5 567-634 bp 1.0 70.4
rpl23-trnI-CAU_p3 CCCAATGCTTTATCTCTGTCCT TGGCGAATTCGTAGGTTCAA 45.5 45.0 57.8 57.5 565-632 bp 1.0 70.0
rpl23-trnI-CAU_p4 CCCAATGCTTTATCTCTGTCCT TTGGCGAATTCGTAGGTTCA 45.5 45.0 57.8 57.5 566-633 bp 1.0 70.0
rpl23-trnI-CAU_p5 TCGACCCAATGCTTTATCTCT TGGCGAATTCGTAGGTTCAA 42.9 45.0 56.8 57.5 569-636 bp 1.0 69.7