Hotspot

psbN-psbH

LSC · 78424-78536 · 113 bp

Back to Corchorus

Pi 0.0442

MarkerSeek score 74.30

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 5
pi: 0.0442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GCGACTAAGGTTGCTGTTTC	TCCCCTACAACAGTTCGTCT	50.0	50.0	57.4	58.0	198 bp	1.0	77.2
psbN-psbH_p2	TAGTTGTTGAGAGGGTTGCC	TCCCCTACAACAGTTCGTCT	50.0	50.0	57.4	58.0	277 bp	1.0	76.9
psbN-psbH_p3	GCGACTAAGGTTGCTGTTTC	ACAACAGTTCGTCTTGGACC	50.0	50.0	57.4	58.1	192 bp	1.0	76.9
psbN-psbH_p4	TAGTTGTTGAGAGGGTTGCC	ACAACAGTTCGTCTTGGACC	50.0	50.0	57.4	58.1	271 bp	1.0	76.6
psbN-psbH_p5	TAGTTGTTGAGAGGGTTGCC	CAACAGTTCGTCTTGGACCA	50.0	50.0	57.4	58.1	270 bp	1.0	76.6