Hotspot

rps12-clpP

LSC · 73707-73889 · 183 bp

Back to Corchorus

Pi 0.331

MarkerSeek score 75.30

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 53
pi: 0.331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACCCTGTTAGTCCGTTCT	TATAAAGCAACGGGGCCATC	50.0	50.0	58.0	58.0	1051-1097 bp	1.0	91.1
rps12-clpP_p2	TGACCCTGTTAGTCCGTTCT	CGAAGACCTGGAAAGGGATG	50.0	55.0	58.0	58.0	680-719 bp	1.0	90.9
rps12-clpP_p3	CTAGTACATGTTCCTCGGCG	TATAAAGCAACGGGGCCATC	55.0	50.0	57.9	58.0	746-792 bp	1.0	90.8
rps12-clpP_p4	TGACCCTGTTAGTCCGTTCT	CCCATTTCAGCGTCACAAAC	50.0	50.0	58.0	57.9	1242 bp	0.5	59.6
rps12-clpP_p5	TGACCCTGTTAGTCCGTTCT	TCCTCGCCATTCGTGAAAAA	50.0	45.0	58.0	58.1	750 bp	0.5	59.0