Hotspot

rbcL-psaI

LSC · 59758-62633 · 2876 bp

Back to Corchorus

Pi 0.174

MarkerSeek score 79.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 2876
variable_sites: 428
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GAGGCTAGCAAATGGAGTCC	AAAAATAGAGGGGAAGCCGC	55.0	50.0	58.0	58.2	2999 bp	0.5	57.8
rbcL-psaI_p2	GAGGCTAGCAAATGGAGTCC	AAGCCGCTGAAAGTTGTCAT	55.0	45.0	58.0	58.4	2986 bp	0.5	56.6
rbcL-psaI_p3	GAGTCCTGAACTAGCTGCTG	AAAAATAGAGGGGAAGCCGC	55.0	50.0	57.7	58.2	2985 bp	0.5	55.8
rbcL-psaI_p4	GGCTAGCAAATGGAGTCCTG	AAAAATAGAGGGGAAGCCGC	55.0	50.0	58.3	58.2	2997 bp	0.5	55.5
rbcL-psaI_p5	AGGCTAGCAAATGGAGTCCT	AAAAATAGAGGGGAAGCCGC	50.0	50.0	58.4	58.2	2998 bp	0.5	54.8