primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CTGCCGATGGTATGAACGAA	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.1	58.0	0.0	17.2	0.078529	4379	5273	1026-1087	1.000000	84.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TGGTCTTACAAACTCTGCCG	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	57.8	58.0	0.0	17.2	0.176936	4379	5273	1040-1101	1.000000	83.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4379	5273	962-1023	1.000000	83.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4379	5273	961-1022	1.000000	83.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.3	58.0	5.3	17.2	0.295924	4379	5273	971-1032	1.000000	82.7
rbcL-psaI_p1	rbcL-psaI	1	GAGGCTAGCAAATGGAGTCC	AAAAATAGAGGGGAAGCCGC	20	20	55.0	50.0	58.0	58.2	18.2	0.0	0.275615	59758	62633	2999-2999	0.500000	57.8
rbcL-psaI_p2	rbcL-psaI	2	GAGGCTAGCAAATGGAGTCC	AAGCCGCTGAAAGTTGTCAT	20	20	55.0	45.0	58.0	58.4	18.2	0.0	0.430375	59758	62633	2986-2986	0.500000	56.6
rbcL-psaI_p3	rbcL-psaI	3	GAGTCCTGAACTAGCTGCTG	AAAAATAGAGGGGAAGCCGC	20	20	55.0	50.0	57.7	58.2	13.4	0.0	0.528135	59758	62633	2985-2985	0.500000	55.8
rbcL-psaI_p4	rbcL-psaI	4	GGCTAGCAAATGGAGTCCTG	AAAAATAGAGGGGAAGCCGC	20	20	55.0	50.0	58.3	58.2	18.2	0.0	0.562864	59758	62633	2997-2997	0.500000	55.5
rbcL-psaI_p5	rbcL-psaI	5	AGGCTAGCAAATGGAGTCCT	AAAAATAGAGGGGAAGCCGC	20	20	50.0	50.0	58.4	58.2	18.2	0.0	0.646618	59758	62633	2998-2998	0.500000	54.8
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	69844	70047	264-272	1.000000	83.9
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	69844	70047	262-270	1.000000	83.9
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	69844	70047	265-273	1.000000	83.9
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	69844	70047	275-283	1.000000	83.8
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	69844	70047	276-284	1.000000	83.8
rps18_p1	rps18	1	ACCCTTCCCGAGAGTATGAG	CTTTTTCTTCCCCAACCCGA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.085022	71727	72095	892-969	1.000000	83.1
rps18_p2	rps18	2	ACCCTTCCCGAGAGTATGAG	TCTTTTTCTTCCCCAACCCG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.085022	71727	72095	893-970	1.000000	83.1
rps18_p3	rps18	3	AAAGGAGGTTCATGGCCAAG	CTTTTTCTTCCCCAACCCGA	20	20	50.0	50.0	58.1	58.0	11.0	0.0	0.081426	71727	72095	1127-1204	1.000000	82.4
rps18_p4	rps18	4	AAAGGAGGTTCATGGCCAAG	TCTTTTTCTTCCCCAACCCG	20	20	50.0	50.0	58.1	58.0	11.0	0.0	0.081426	71727	72095	1128-1205	1.000000	82.4
rps18_p5	rps18	5	AAAGGAGGTTCATGGCCAAG	GGGTTCCCTCGAGAATGAAC	20	20	50.0	55.0	58.1	58.0	11.0	23.1	0.096720	71727	72095	1248-1327	1.000000	82.0
rpl20_p1	rpl20	1	AGACAATTCCTATTTGAGATAGCCA	CTGAAAGAAAGAATTAGTTATTCATCA	25	27	36.0	25.9	57.9	54.6	0.0	4.8	15.539745	72457	72822	446-446	0.500000	20.0
rpl20_p2	rpl20	2	AGACAATTCCTATTTGAGATAGCCA	ACTGAAAGAAAGAATTAGTTATTCATC	25	27	36.0	25.9	57.9	54.6	0.0	4.8	15.542451	72457	72822	447-447	0.500000	20.0
rpl20_p3	rpl20	3	TCCTATTTGAGATAGCCAAGT	CTGAAAGAAAGAATTAGTTATTCATCA	21	27	38.1	25.9	53.7	54.6	0.0	4.8	15.666233	72457	72822	439-439	0.500000	20.0
rpl20_p4	rpl20	4	TCCTATTTGAGATAGCCAAGT	ACTGAAAGAAAGAATTAGTTATTCATC	21	27	38.1	25.9	53.7	54.6	0.0	4.8	15.668939	72457	72822	440-440	0.500000	20.0
rpl20_p5	rpl20	5	TTCCTATTTGAGATAGCCAAGT	CTGAAAGAAAGAATTAGTTATTCATCA	22	27	36.4	25.9	54.6	54.6	0.0	4.8	15.809374	72457	72822	440-440	0.500000	20.0
rps12-clpP_p1	rps12-clpP	1	TGACCCTGTTAGTCCGTTCT	TATAAAGCAACGGGGCCATC	20	20	50.0	50.0	58.0	58.0	0.0	4.4	0.029711	73707	73889	1051-1097	1.000000	91.1
rps12-clpP_p2	rps12-clpP	2	TGACCCTGTTAGTCCGTTCT	CGAAGACCTGGAAAGGGATG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.035208	73707	73889	680-719	1.000000	90.9
rps12-clpP_p3	rps12-clpP	3	CTAGTACATGTTCCTCGGCG	TATAAAGCAACGGGGCCATC	20	20	55.0	50.0	57.9	58.0	2.3	4.4	0.145457	73707	73889	746-792	1.000000	90.8
rps12-clpP_p4	rps12-clpP	4	TGACCCTGTTAGTCCGTTCT	CCCATTTCAGCGTCACAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.055847	73707	73889	1242-1242	0.500000	59.6
rps12-clpP_p5	rps12-clpP	5	TGACCCTGTTAGTCCGTTCT	TCCTCGCCATTCGTGAAAAA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.119810	73707	73889	750-750	0.500000	59.0
clpP_p1	clpP	1	TGACCCTGTTAGTCCGTTCT	CGCAATGGGGGTTGATTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.056705	73890	75982	2931-2991	1.000000	89.8
clpP_p2	clpP	2	CTAGTACATGTTCCTCGGCG	CGCAATGGGGGTTGATTCTA	20	20	55.0	50.0	57.9	57.9	2.3	0.0	0.172451	73890	75982	2626-2686	1.000000	89.5
clpP_p3	clpP	3	AGAGGGCAAAGAGGTAGAGG	TGCCCCCTGTAATACTCCAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.153199	73890	75982	2892-2892	0.500000	58.8
clpP_p4	clpP	4	AGAGGTAGAGGGCAAAGAGG	TGCCCCCTGTAATACTCCAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.153199	73890	75982	2898-2898	0.500000	58.8
clpP_p5	clpP	5	AGAGGGCAAAGAGGTAGAGG	CTGTAATACTCCAACCGCCC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.177762	73890	75982	2886-2886	0.500000	58.6
psbN-psbH_p1	psbN-psbH	1	GCGACTAAGGTTGCTGTTTC	TCCCCTACAACAGTTCGTCT	20	20	50.0	50.0	57.4	58.0	1.6	0.0	0.601634	78424	78536	198-198	1.000000	77.2
psbN-psbH_p2	psbN-psbH	2	TAGTTGTTGAGAGGGTTGCC	TCCCCTACAACAGTTCGTCT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.566848	78424	78536	277-277	1.000000	76.9
psbN-psbH_p3	psbN-psbH	3	GCGACTAAGGTTGCTGTTTC	ACAACAGTTCGTCTTGGACC	20	20	50.0	50.0	57.4	58.1	1.6	0.0	0.646798	78424	78536	192-192	1.000000	76.9
psbN-psbH_p4	psbN-psbH	4	TAGTTGTTGAGAGGGTTGCC	ACAACAGTTCGTCTTGGACC	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.612013	78424	78536	271-271	1.000000	76.6
psbN-psbH_p5	psbN-psbH	5	TAGTTGTTGAGAGGGTTGCC	CAACAGTTCGTCTTGGACCA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.613374	78424	78536	270-270	1.000000	76.6
rps11_p1	rps11	1	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGA	20	21	50.0	33.3	58.0	52.8	10.8	0.0	6.229148	83090	83506	604-622	1.000000	42.2
rps11_p2	rps11	2	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGAA	20	22	50.0	31.8	58.0	53.7	10.8	0.0	6.340276	83090	83506	604-622	1.000000	42.2
rps11_p3	rps11	3	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGAAA	20	23	50.0	30.4	58.0	54.5	10.8	0.0	6.528654	83090	83506	604-622	1.000000	42.2
rps11_p4	rps11	4	TTCCACTGTAGTGTCCGAGT	GAAGATCTGTTTTGACATGAA	20	21	50.0	33.3	58.0	52.1	10.8	0.0	6.925117	83090	83506	603-621	1.000000	42.2
rps11_p5	rps11	5	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGAAAT	20	24	50.0	29.2	58.0	55.0	10.8	0.0	6.983668	83090	83506	604-622	1.000000	42.2
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	ACCCAATGCTTTATCTCTGTCC	TGGCGAATTCGTAGGTTCAA	22	20	45.5	45.0	58.1	57.5	0.0	27.2	2.568012	90494	90975	566-633	1.000000	70.4
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	ACCCAATGCTTTATCTCTGTCC	TTGGCGAATTCGTAGGTTCA	22	20	45.5	45.0	58.1	57.5	0.0	27.2	2.568012	90494	90975	567-634	1.000000	70.4
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	CCCAATGCTTTATCTCTGTCCT	TGGCGAATTCGTAGGTTCAA	22	20	45.5	45.0	57.8	57.5	0.0	27.2	2.621101	90494	90975	565-632	1.000000	70.0
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	CCCAATGCTTTATCTCTGTCCT	TTGGCGAATTCGTAGGTTCA	22	20	45.5	45.0	57.8	57.5	0.0	27.2	2.621101	90494	90975	566-633	1.000000	70.0
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	TCGACCCAATGCTTTATCTCT	TGGCGAATTCGTAGGTTCAA	21	20	42.9	45.0	56.8	57.5	0.0	27.2	2.651992	90494	90975	569-636	1.000000	69.7
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GGTCCCACTCTTCTACCCTT	GTTGAACCCTGTGAATTGCG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.096442	91050	91192	1020-1111	1.000000	89.6
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GGTCCCACTCTTCTACCCTT	CCAGCGACGGTTTCATTAGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.050547	91050	91192	1410-1501	1.000000	89.1
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GGTCCCACTCTTCTACCCTT	TCCAGCGACGGTTTCATTAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.050547	91050	91192	1411-1502	1.000000	89.1
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GAAGAGTTCGACCCAATGCT	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.105187	91050	91192	1345-1436	1.000000	89.0
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GGTCCCACTCTTCTACCCTT	AATCATGGTCCTTGCGGATC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.073093	91050	91192	1502-1593	1.000000	88.7
ycf1_p1	ycf1	1	CATGATGCAGACAACGAGGA	GTTTCCCTTCCCCAAACTGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.096476	117293	117412	986-986	0.500000	59.2
ycf1_p2	ycf1	2	AGAGCAGAGCCTCAAATTCG	GTTTCCCTTCCCCAAACTGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.103495	117293	117412	959-959	0.500000	59.2
ycf1_p3	ycf1	3	CATGATGCAGACAACGAGGA	TACCGTCCGTTAACCAGTCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.104636	117293	117412	829-829	0.500000	59.2
ycf1_p4	ycf1	4	AGAGCAGAGCCTCAAATTCG	TACCGTCCGTTAACCAGTCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.111655	117293	117412	802-802	0.500000	59.1
ycf1_p5	ycf1	5	TAGAACGCCATGATGCAGAC	GTTTCCCTTCCCCAAACTGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.142166	117293	117412	994-994	0.500000	58.9
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GTTGAACCCTGTGAATTGCG	GGTCCCACTCTTCTACCCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.096442	158512	158654	1020-1111	1.000000	89.6
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	CCAGCGACGGTTTCATTAGA	GGTCCCACTCTTCTACCCTT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.050547	158512	158654	1410-1501	1.000000	89.1
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	TCCAGCGACGGTTTCATTAG	GGTCCCACTCTTCTACCCTT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.050547	158512	158654	1411-1502	1.000000	89.1
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CCAGCGACGGTTTCATTAGA	GAAGAGTTCGACCCAATGCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.105187	158512	158654	1345-1436	1.000000	89.0
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	AATCATGGTCCTTGCGGATC	GGTCCCACTCTTCTACCCTT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.073093	158512	158654	1502-1593	1.000000	88.7
trnI-CAU-rpl23_p1	trnI-CAU-rpl23	1	TGGCGAATTCGTAGGTTCAA	ACCCAATGCTTTATCTCTGTCC	20	22	45.0	45.5	57.5	58.1	27.2	0.0	2.568012	158729	159210	566-633	1.000000	70.4
trnI-CAU-rpl23_p2	trnI-CAU-rpl23	2	TTGGCGAATTCGTAGGTTCA	ACCCAATGCTTTATCTCTGTCC	20	22	45.0	45.5	57.5	58.1	27.2	0.0	2.568012	158729	159210	567-634	1.000000	70.4
trnI-CAU-rpl23_p3	trnI-CAU-rpl23	3	TGGCGAATTCGTAGGTTCAA	CCCAATGCTTTATCTCTGTCCT	20	22	45.0	45.5	57.5	57.8	27.2	0.0	2.621101	158729	159210	565-632	1.000000	70.0
trnI-CAU-rpl23_p4	trnI-CAU-rpl23	4	TTGGCGAATTCGTAGGTTCA	CCCAATGCTTTATCTCTGTCCT	20	22	45.0	45.5	57.5	57.8	27.2	0.0	2.621101	158729	159210	566-633	1.000000	70.0
trnI-CAU-rpl23_p5	trnI-CAU-rpl23	5	TGGCGAATTCGTAGGTTCAA	TCGACCCAATGCTTTATCTCT	20	21	45.0	42.9	57.5	56.8	27.2	0.0	2.651992	158729	159210	569-636	1.000000	69.7