Hotspot

ndhA

SSC · 124949-127195 · 2247 bp

Back to Corchoropsis

Pi 0.00238

MarkerSeek score 59.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 8
pi: 0.00238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	47.6	50.0	58.1	58.5	2461-2478 bp	1.0	67.7
ndhA_p2	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAAT	47.6	50.0	57.8	58.5	2464-2481 bp	1.0	66.7
ndhA_p3	TGGCCATAATTCATGAACCCA	GCCCACCGGGTTTTATCAAT	42.9	50.0	57.6	58.5	2477-2494 bp	1.0	65.2
ndhA_p4	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	47.6	52.6	58.1	58.0	2461-2478 bp	1.0	63.6
ndhA_p5	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAA	47.6	52.6	57.8	58.0	2464-2481 bp	1.0	62.5