Hotspot

ycf4-cemA

LSC · 63958-64954 · 997 bp

Back to Corchoropsis

Pi 0.000669

MarkerSeek score 59.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 1
pi: 0.000669
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTCCCTTGACTCGTACTG	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.0	58.1	1170-1172 bp	1.0	79.4
ycf4-cemA_p2	TGCGTGTACCGATTGAAGTT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1093-1095 bp	1.0	78.3
ycf4-cemA_p3	TTGCGTGTACCGATTGAAGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1094-1096 bp	1.0	78.3
ycf4-cemA_p4	CGTTCCCTTGACTCGTACTG	GAGAGAGAGATCCACCAGGG	55.0	60.0	58.0	58.3	1176-1178 bp	1.0	77.7
ycf4-cemA_p5	TGCGTGTACCGATTGAAGTT	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.8	58.3	1099-1101 bp	1.0	76.6