Hotspot

accD-psaI

LSC · 62162-62896 · 735 bp

Back to Corchoropsis

Pi 0.000908

MarkerSeek score 59.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 1
pi: 0.000908
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTAGTTCAGCTCCACGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	877-880 bp	1.0	80.1
accD-psaI_p2	AGTTCAGCTCCACGGTTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	872-875 bp	1.0	79.7
accD-psaI_p3	GAGTTAGTTCAGCTCCACGG	GAGGGAAAGCTGCTGAAAGT	55.0	50.0	58.0	58.1	830-833 bp	1.0	79.6
accD-psaI_p4	GAGTTAGTTCAGCTCCACGG	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	883-886 bp	1.0	79.5
accD-psaI_p5	AGTTCAGCTCCACGGTTTTT	GAGGGAAAGCTGCTGAAAGT	45.0	50.0	57.9	58.1	825-828 bp	1.0	79.2