Hotspot

ndhC-trnV-UAC

LSC · 53424-54602 · 1179 bp

Back to Corchoropsis

Pi 0.00792

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 14
pi: 0.00792
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1323-1346 bp	1.0	77.9
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1311-1334 bp	1.0	77.8
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1313-1336 bp	1.0	76.0
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1314-1337 bp	1.0	76.0
ndhC-trnV-UAC_p5	ACCCTTTCTAATCGGGGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.8	57.5	1322-1345 bp	1.0	75.1