Hotspot

trnF-GAA-ndhJ

LSC · 51026-51699 · 674 bp

Back to Corchoropsis

Pi 0.000992
MarkerSeek score 65.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
674
variable_sites
1
pi
0.000992
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA ATCATCCACGACTGAAACGT 50.0 45.0 58.1 57.3 805-916 bp 1.0 77.0
trnF-GAA-ndhJ_p2 TAATGCGTCGGTAATGGTCG ATCATCCACGACTGAAACGT 50.0 45.0 58.2 57.3 860-971 bp 1.0 76.0
trnF-GAA-ndhJ_p3 AATGGTCGGGATAGCTCAGT ATCATCCACGACTGAAACGT 50.0 45.0 58.2 57.3 848-959 bp 1.0 75.7
trnF-GAA-ndhJ_p4 ATGGTCGGGATAGCTCAGTT ATCATCCACGACTGAAACGT 50.0 45.0 58.2 57.3 847-958 bp 1.0 75.7
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA AAGGATTACATTGCCCCCAA 50.0 45.0 58.1 57.1 746-857 bp 1.0 75.7