Hotspot

trnG-GCC-trnfM-CAU

LSC · 38338-38501 · 164 bp

Back to Corchoropsis

Pi 0.0285

MarkerSeek score 61.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 7
pi: 0.0285
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	TTTTCATCGCGGGATAGAGC	50.0	50.0	57.8	58.1	289-290 bp	1.0	79.5
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	263-264 bp	1.0	79.0
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	262-263 bp	1.0	79.0
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	AGGTCATGGGTTCAAATCCC	50.0	50.0	57.8	57.5	238-239 bp	1.0	76.7
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	CCTTGAGGTCATGGGTTCAA	50.0	50.0	57.8	57.4	243-244 bp	1.0	76.3