Hotspot

psbZ-trnG-GCC

LSC · 37494-38266 · 773 bp

Back to Corchoropsis

Pi 0.00517

MarkerSeek score 54.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 6
pi: 0.00517
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	930-933 bp	1.0	78.5
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	928-931 bp	1.0	78.5
psbZ-trnG-GCC_p3	CTCCTGATGGTTGGTTGAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	924-927 bp	1.0	73.3
psbZ-trnG-GCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	929-932 bp	1.0	72.7
psbZ-trnG-GCC_p5	TCTCCTGATGGTTGGTTGAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	925-928 bp	1.0	70.9