Hotspot

petN-psbM

LSC · 29532-30319 · 788 bp

Back to Corchoropsis

Pi 0.00338

MarkerSeek score 61.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 4
pi: 0.00338
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	GCTACAGCGCTCTTCATTCT	45.0	50.0	58.0	58.1	942-949 bp	1.0	80.0
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TTGCTACAGCGCTCTTCATT	45.0	45.0	58.0	57.9	944-951 bp	1.0	79.8
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	GCTACAGCGCTCTTCATTCT	50.0	50.0	57.8	58.1	948-955 bp	1.0	78.5
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	TTGCTACAGCGCTCTTCATT	50.0	45.0	57.8	57.9	950-957 bp	1.0	78.3
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	ATTGCTACAGCGCTCTTCAT	45.0	45.0	58.0	57.7	945-952 bp	1.0	78.0