Hotspot

ndhA

SSC · 92539-92987 · 449 bp

Back to Corallorhiza

Pi 0.192

MarkerSeek score 70.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 449
variable_sites: 207
pi: 0.192
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AAATAAGGAACTGCGTGGCA	TATCGCAAAGGGGTCTACGA	45.0	50.0	58.1	58.2	1068-2302 bp	0.384615	64.8
ndhA_p2	TTTTACCCAAGGTTTCGCCA	TATCGCAAAGGGGTCTACGA	45.0	50.0	57.9	58.2	1375-2749 bp	0.230769	58.6
ndhA_p3	TTTTACCCAAGGTTTCGCCA	AGCGGGTCTGTTGAATTTCA	45.0	45.0	57.9	57.7	1463-2837 bp	0.230769	58.0
ndhA_p4	ACTGAAATGCATCAACCCGT	TATCGCAAAGGGGTCTACGA	45.0	50.0	58.1	58.2	1341-1342 bp	0.153846	43.6
ndhA_p5	ACTGAAATGCATCAACCCGT	AGCGGGTCTGTTGAATTTCA	45.0	45.0	58.1	57.7	1429-1430 bp	0.153846	43.3