Hotspot

rpl32-trnL-UAG

SSC · 90761-91396 · 636 bp

Back to Corallorhiza

Pi 0.0506

MarkerSeek score 81.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 636
variable_sites: 105
pi: 0.0506
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	703-766 bp	1.0	90.7
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	696-759 bp	1.0	90.2
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	920-1297 bp	0.923077	88.1
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	ACGGGTTGATGCATTTCAGT	50.0	45.0	58.0	58.1	1241-1291 bp	0.461538	69.0
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	GGCAGGATCACTTCATTGGT	50.0	50.0	58.0	57.9	1442-1452 bp	0.153846	44.8