Hotspot

ycf1-rpl32

SSC · 90141-90586 · 446 bp

Back to Corallorhiza

Pi 0.166

MarkerSeek score 76.40

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 446
variable_sites: 188
pi: 0.166
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	CAAGTTCCACGTTAGCCAGA	CTTTTTGACTGTCCGGTGGA	50.0	50.0	57.8	58.0	912-1406 bp	0.769231	81.2
ycf1-rpl32_p2	CCTCAGAAACGGAAGAAGGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	57.9	58.0	612-1047 bp	0.538462	72.4
ycf1-rpl32_p3	CTCAGAAACGGAAGAAGGGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	57.9	58.0	611-1046 bp	0.538462	72.4
ycf1-rpl32_p4	CCGCCCAGTTTATCGACTTT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.3	58.0	238-682 bp	0.384615	65.0
ycf1-rpl32_p5	TCAGAAACGGAAGAAGGGGA	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.3	58.0	964 bp	0.153846	43.7