Hotspot

clpP-psbB

LSC · 55255-55830 · 576 bp

Back to Corallorhiza

Pi 0.048

MarkerSeek score 84.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 576
variable_sites: 83
pi: 0.048
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACTCTTGGAACACCAATGGG	CACGATACCAAGGTAAACCCA	50.0	47.6	57.7	57.7	621-825 bp	1.0	79.0
clpP-psbB_p2	ACTCTTGGAACACCAATGGG	ACACGATACCAAGGTAAACCC	50.0	47.6	57.7	57.7	622-826 bp	1.0	78.9
clpP-psbB_p3	CTTGGAACACCAATGGGCAT	CACGATACCAAGGTAAACCCA	50.0	47.6	58.7	57.7	618-822 bp	1.0	75.5
clpP-psbB_p4	CTTGGAACACCAATGGGCAT	ACACGATACCAAGGTAAACCC	50.0	47.6	58.7	57.7	619-823 bp	1.0	75.4
clpP-psbB_p5	ACTCTTGGAACACCAATGGG	ACGATACCAAGGTAAACCCA	50.0	45.0	57.7	56.2	620-824 bp	1.0	74.9