Hotspot

accD-psaI

LSC · 46785-47321 · 537 bp

Back to Corallorhiza

Pi 0.0486

MarkerSeek score 78.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 76
pi: 0.0486
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATGCCTACATTGCGTTTGC	AACTAGCAGCATTCCGACAG	45.0	50.0	58.0	58.0	1117-1476 bp	0.769231	81.0
accD-psaI_p2	CCTACAACTGGCGGAGTTAC	AACTAGCAGCATTCCGACAG	55.0	50.0	58.0	58.0	1180-1539 bp	0.769231	80.8
accD-psaI_p3	CACGGTTTCCTTCCCTTGAA	TCAACTAGCAGCATTCCGAC	50.0	50.0	58.0	58.0	1004-1313 bp	0.692308	78.0
accD-psaI_p4	AATGCCTACATTGCGTTTGC	TCAACTAGCAGCATTCCGAC	45.0	50.0	58.0	58.0	1169-1478 bp	0.692308	77.3
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TCAACTAGCAGCATTCCGAC	55.0	50.0	58.0	58.0	1232-1541 bp	0.692308	76.9