Hotspot

atpB-rbcL

LSC · 42312-43213 · 902 bp

Back to Corallorhiza

Pi 0.0581

MarkerSeek score 76.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 125
pi: 0.0581
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGCCCGGTGTAAAAATGACA	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	1088-1663 bp	1.0	91.8
atpB-rbcL_p2	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1475-2050 bp	0.923077	88.3
atpB-rbcL_p3	TGCCCGGTGTAAAAATGACA	ACGACCTCGATATGGTAGCA	45.0	50.0	58.0	58.0	1123-1286 bp	0.538462	72.6
atpB-rbcL_p4	AGGGGGCTAAAAGATCCACT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1707-2032 bp	0.384615	61.8
atpB-rbcL_p5	AGGGGGCTAAAAGATCCACT	ACGACCTCGATATGGTAGCA	50.0	50.0	58.0	58.0	1622-1655 bp	0.153846	47.5