Hotspot

psbM-trnD-GUC

LSC · 21972-23056 · 1085 bp

Back to Corallorhiza

Pi 0.0508

MarkerSeek score 83.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 180
pi: 0.0508
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	980-1231 bp	0.461538	64.3
psbM-trnD-GUC_p2	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1032-1283 bp	0.461538	64.2
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	AAACAGAAATCCCGGATCCG	45.0	50.0	58.3	58.0	1496-1541 bp	0.384615	55.8
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	AAACAGAAATCCCGGATCCG	45.0	50.0	58.3	58.0	1444-1489 bp	0.384615	55.8
psbM-trnD-GUC_p5	TGAACAGCGCAGTAGCAATA	AAACAGAAATCCCGGATCCG	45.0	50.0	57.6	58.0	1443-1488 bp	0.384615	54.8