Hotspot

trnS-GCU-trnG-UCC

LSC · 7651-8410 · 760 bp

Back to Corallorhiza

Pi 0.0556
MarkerSeek score 82.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
760
variable_sites
136
pi
0.0556
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 815-856 bp 1.0 59.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 814-855 bp 1.0 59.9
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 816-857 bp 1.0 58.8
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 815-856 bp 1.0 58.8
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TTTACCACTAAACTATACCCGC 50.0 40.9 58.0 55.6 817-858 bp 1.0 57.1