Hotspot

rps16-trnQ-UUG

LSC · 5488-6443 · 956 bp

Back to Corallorhiza

Pi 0.0679

MarkerSeek score 84.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 250
pi: 0.0679
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CTACGTCGAGACAAGAGCAC	GACCAAATCAAAATGGGGCG	55.0	50.0	58.1	58.0	1041-1250 bp	1.0	91.0
rps16-trnQ-UUG_p2	CTACGTCGAGACAAGAGCAC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	980-1189 bp	1.0	90.5
rps16-trnQ-UUG_p3	CTACGTCGAGACAAGAGCAC	CAAGTGGTAAGGCAATGGGT	55.0	50.0	58.1	58.1	1017-1226 bp	1.0	90.5
rps16-trnQ-UUG_p4	CCGGTTCCTAGCAGAACAAA	GACCAAATCAAAATGGGGCG	50.0	50.0	57.8	58.0	1061-1216 bp	0.461538	67.2
rps16-trnQ-UUG_p5	AGATCCGGTTCCTAGCAGAA	GACCAAATCAAAATGGGGCG	50.0	50.0	57.8	58.0	1160-1220 bp	0.384615	61.6