primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGAATTCTTGTGCCCCCAAG	TCGATCCCGAAGAGAAGGAA	20	20	50.0	50.0	58.1	57.9	0.9	0.0	0.210455	2806	3830	1888-2093	0.692308	78.0
matK-trnK-UUU_p2	matK-trnK-UUU	2	GAATTCTTGTGCCCCCAAGA	TCGATCCCGAAGAGAAGGAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.215968	2806	3830	1887-2092	0.692308	78.0
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGAATTCTTGTGCCCCCAAG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.1	58.2	0.9	17.1	0.239552	2806	3830	1258-1373	0.692308	77.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	AGAATTCTTGTGCCCCCAAG	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	58.1	57.9	0.9	0.0	0.180557	2806	3830	1276-1391	0.461538	67.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	GAATTCTTGTGCCCCCAAGA	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.186069	2806	3830	1275-1390	0.461538	67.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CTACGTCGAGACAAGAGCAC	GACCAAATCAAAATGGGGCG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.123996	5488	6443	1041-1250	1.000000	91.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CTACGTCGAGACAAGAGCAC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.1	58.1	0.0	16.9	0.191345	5488	6443	980-1189	1.000000	90.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CTACGTCGAGACAAGAGCAC	CAAGTGGTAAGGCAATGGGT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.191888	5488	6443	1017-1226	1.000000	90.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCGGTTCCTAGCAGAACAAA	GACCAAATCAAAATGGGGCG	20	20	50.0	50.0	57.8	58.0	14.0	0.0	0.187664	5488	6443	1061-1216	0.461538	67.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGATCCGGTTCCTAGCAGAA	GACCAAATCAAAATGGGGCG	20	20	50.0	50.0	57.8	58.0	9.0	0.0	0.169472	5488	6443	1160-1220	0.384615	61.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TACCACTAAACTATACCCGC	20	20	50.0	45.0	58.0	54.0	0.0	0.0	4.015659	7651	8410	815-856	1.000000	59.9
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	TACCACTAAACTATACCCGC	20	20	50.0	45.0	58.0	54.0	0.0	0.0	4.017047	7651	8410	814-855	1.000000	59.9
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	TTACCACTAAACTATACCCGC	20	21	50.0	42.9	58.0	54.9	0.0	0.0	4.151919	7651	8410	816-857	1.000000	58.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	TTACCACTAAACTATACCCGC	20	21	50.0	42.9	58.0	54.9	0.0	0.0	4.153307	7651	8410	815-856	1.000000	58.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	TTTACCACTAAACTATACCCGC	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.365806	7651	8410	817-858	1.000000	57.1
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9183	9327	225-237	1.000000	73.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9183	9327	228-240	1.000000	73.2
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9183	9327	231-243	1.000000	72.7
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9183	9327	234-246	1.000000	72.4
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCAACGTCGACTATAACCCC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.8	56.9	14.7	0.0	2.265991	9183	9327	246-258	1.000000	71.8
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.3	57.9	11.5	0.0	0.343293	21972	23056	980-1231	0.461538	64.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.340822	21972	23056	1032-1283	0.461538	64.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TTGGCTGACTGTTTTGACGT	AAACAGAAATCCCGGATCCG	20	20	45.0	50.0	58.3	58.0	0.0	27.6	0.293155	21972	23056	1496-1541	0.384615	55.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	ATGAACAGCGCAGTAGCAAT	AAACAGAAATCCCGGATCCG	20	20	45.0	50.0	58.3	58.0	11.5	27.6	0.295626	21972	23056	1444-1489	0.384615	55.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TGAACAGCGCAGTAGCAATA	AAACAGAAATCCCGGATCCG	20	20	45.0	50.0	57.6	58.0	11.5	27.6	0.416832	21972	23056	1443-1488	0.384615	54.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAAAAAGAGCCGGCTATCG	GAGTCTCTGCACCTATCCCT	20	20	50.0	55.0	58.1	57.9	33.0	0.0	0.139329	34888	35477	802-871	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AGAAAAAGAGCCGGCTATCG	GCTTCCATTGAGTCTCTGCA	20	20	50.0	50.0	58.1	57.9	33.0	0.6	0.163681	34888	35477	811-880	1.000000	90.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAAAACCTGGCCTGTTGACC	GAGTCTCTGCACCTATCCCT	20	20	50.0	55.0	58.0	57.9	3.3	0.0	0.090948	34888	35477	1379-1448	0.923077	88.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TAAAACCTGGCCTGTTGACC	GCTTCCATTGAGTCTCTGCA	20	20	50.0	50.0	58.0	57.9	3.3	0.6	0.115300	34888	35477	1388-1457	0.923077	88.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAAAACCTGGCCTGTTGACC	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	58.0	58.2	3.3	10.2	0.161197	34888	35477	1261-1262	0.307692	54.6
trnL-UAA_p1	trnL-UAA	1	AGAAAAAGAGCCGGCTATCG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.1	58.1	33.0	20.1	0.110571	35478	36392	1816-2106	1.000000	91.1
trnL-UAA_p2	trnL-UAA	2	AGAAAAAGAGCCGGCTATCG	GAGCGAGGGAGTAAAATGGG	20	20	50.0	55.0	58.1	58.0	33.0	0.0	0.103748	35478	36392	1430-1683	0.923077	88.1
trnL-UAA_p3	trnL-UAA	3	AGAAAAAGAGCCGGCTATCG	GCTATCCCGACCATTTCCTG	20	20	50.0	55.0	58.1	58.1	33.0	0.0	0.174440	35478	36392	1768-2058	0.923077	87.5
trnL-UAA_p4	trnL-UAA	4	AGAAAAAGAGCCGGCTATCG	CCTGTGCATCATCCTAGCAG	20	20	50.0	55.0	58.1	58.1	33.0	0.0	0.178404	35478	36392	1746-2042	0.923077	87.5
trnL-UAA_p5	trnL-UAA	5	AGAAAAAGAGCCGGCTATCG	GAGCTATCCCGACCATTTCC	20	20	50.0	55.0	58.1	57.8	33.0	0.0	0.230016	35478	36392	1770-2060	0.923077	87.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGGTTCAGGCATTTGCTCAT	TGCGCCAATGTTTTTCAAGG	20	20	45.0	45.0	58.1	58.1	2.1	4.2	0.209246	38728	39319	1671-2036	0.769231	80.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGTTCAGGCATTTGCTCAT	TTGGTTTGATCCGGTTCAGG	20	20	45.0	50.0	58.1	58.1	2.1	14.6	0.168226	38728	39319	1547-1921	0.692308	76.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ACATCAAAGCTCATTGCCCA	TTGGTTTGATCCGGTTCAGG	20	20	45.0	50.0	58.1	58.1	0.0	14.6	0.168226	38728	39319	1347-1419	0.230769	54.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ACATCAAAGCTCATTGCCCA	TGCGCCAATGTTTTTCAAGG	20	20	45.0	45.0	58.1	58.1	0.0	4.2	0.209246	38728	39319	1471-1534	0.230769	54.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	ACATCAAAGCTCATTGCCCA	CAGTCCATTCAATGCCAGGT	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.229144	38728	39319	1275-1275	0.153846	44.3
atpB-rbcL_p1	atpB-rbcL	1	TGCCCGGTGTAAAAATGACA	AGAAACGATCTCTCCAACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.019671	42312	43213	1088-1663	1.000000	91.8
atpB-rbcL_p2	atpB-rbcL	2	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.020873	42312	43213	1475-2050	0.923077	88.3
atpB-rbcL_p3	atpB-rbcL	3	TGCCCGGTGTAAAAATGACA	ACGACCTCGATATGGTAGCA	20	20	45.0	50.0	58.0	58.0	0.0	0.8	0.040284	42312	43213	1123-1286	0.538462	72.6
atpB-rbcL_p4	atpB-rbcL	4	AGGGGGCTAAAAGATCCACT	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015251	42312	43213	1707-2032	0.384615	61.8
atpB-rbcL_p5	atpB-rbcL	5	AGGGGGCTAAAAGATCCACT	ACGACCTCGATATGGTAGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.8	0.035864	42312	43213	1622-1655	0.153846	47.5
accD-psaI_p1	accD-psaI	1	AATGCCTACATTGCGTTTGC	AACTAGCAGCATTCCGACAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.020820	46785	47321	1117-1476	0.769231	81.0
accD-psaI_p2	accD-psaI	2	CCTACAACTGGCGGAGTTAC	AACTAGCAGCATTCCGACAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021706	46785	47321	1180-1539	0.769231	80.8
accD-psaI_p3	accD-psaI	3	CACGGTTTCCTTCCCTTGAA	TCAACTAGCAGCATTCCGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034485	46785	47321	1004-1313	0.692308	78.0
accD-psaI_p4	accD-psaI	4	AATGCCTACATTGCGTTTGC	TCAACTAGCAGCATTCCGAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.015229	46785	47321	1169-1478	0.692308	77.3
accD-psaI_p5	accD-psaI	5	CCTACAACTGGCGGAGTTAC	TCAACTAGCAGCATTCCGAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016116	46785	47321	1232-1541	0.692308	76.9
clpP-psbB_p1	clpP-psbB	1	ACTCTTGGAACACCAATGGG	CACGATACCAAGGTAAACCCA	20	21	50.0	47.6	57.7	57.7	3.6	2.6	1.630523	55255	55830	621-825	1.000000	79.0
clpP-psbB_p2	clpP-psbB	2	ACTCTTGGAACACCAATGGG	ACACGATACCAAGGTAAACCC	20	21	50.0	47.6	57.7	57.7	3.6	2.6	1.632082	55255	55830	622-826	1.000000	78.9
clpP-psbB_p3	clpP-psbB	3	CTTGGAACACCAATGGGCAT	CACGATACCAAGGTAAACCCA	20	21	50.0	47.6	58.7	57.7	3.6	2.6	2.068683	55255	55830	618-822	1.000000	75.5
clpP-psbB_p4	clpP-psbB	4	CTTGGAACACCAATGGGCAT	ACACGATACCAAGGTAAACCC	20	21	50.0	47.6	58.7	57.7	3.6	2.6	2.070242	55255	55830	619-823	1.000000	75.4
clpP-psbB_p5	clpP-psbB	5	ACTCTTGGAACACCAATGGG	ACGATACCAAGGTAAACCCA	20	20	50.0	45.0	57.7	56.2	3.6	2.6	2.141373	55255	55830	620-824	1.000000	74.9
ycf1-rpl32_p1	ycf1-rpl32	1	CAAGTTCCACGTTAGCCAGA	CTTTTTGACTGTCCGGTGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.202024	90141	90586	912-1406	0.769231	81.2
ycf1-rpl32_p2	ycf1-rpl32	2	CCTCAGAAACGGAAGAAGGG	CTTTTTGACTGTCCGGTGGA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.138811	90141	90586	612-1047	0.538462	72.4
ycf1-rpl32_p3	ycf1-rpl32	3	CTCAGAAACGGAAGAAGGGG	CTTTTTGACTGTCCGGTGGA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.138811	90141	90586	611-1046	0.538462	72.4
ycf1-rpl32_p4	ycf1-rpl32	4	CCGCCCAGTTTATCGACTTT	CTTTTTGACTGTCCGGTGGA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.304044	90141	90586	238-682	0.384615	65.0
ycf1-rpl32_p5	ycf1-rpl32	5	TCAGAAACGGAAGAAGGGGA	CTTTTTGACTGTCCGGTGGA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.313662	90141	90586	964-964	0.153846	43.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.162484	90761	91396	703-766	1.000000	90.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	9.5	0.0	0.223211	90761	91396	696-759	1.000000	90.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	9.5	0.0	0.107041	90761	91396	920-1297	0.923077	88.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCCACCGGACAGTCAAAAAG	ACGGGTTGATGCATTTCAGT	20	20	50.0	45.0	58.0	58.1	9.5	0.0	0.128507	90761	91396	1241-1291	0.461538	69.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCCACCGGACAGTCAAAAAG	GGCAGGATCACTTCATTGGT	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.172995	90761	91396	1442-1452	0.153846	44.8
ndhA_p1	ndhA	1	AAATAAGGAACTGCGTGGCA	TATCGCAAAGGGGTCTACGA	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.329220	92539	92987	1068-2302	0.384615	64.8
ndhA_p2	ndhA	2	TTTTACCCAAGGTTTCGCCA	TATCGCAAAGGGGTCTACGA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.302341	92539	92987	1375-2749	0.230769	58.6
ndhA_p3	ndhA	3	TTTTACCCAAGGTTTCGCCA	AGCGGGTCTGTTGAATTTCA	20	20	45.0	45.0	57.9	57.7	0.0	0.0	0.339186	92539	92987	1463-2837	0.230769	58.0
ndhA_p4	ndhA	4	ACTGAAATGCATCAACCCGT	TATCGCAAAGGGGTCTACGA	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.323807	92539	92987	1341-1342	0.153846	43.6
ndhA_p5	ndhA	5	ACTGAAATGCATCAACCCGT	AGCGGGTCTGTTGAATTTCA	20	20	45.0	45.0	58.1	57.7	0.0	0.0	0.360652	92539	92987	1429-1430	0.153846	43.3