Hotspot

moeB

Genome · 47005-48099 · 1095 bp

Back to Corallina

Pi 0.0475

MarkerSeek score 73.90

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1095
variable_sites: 76
pi: 0.0475
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	AAACACGCCTTGAAGGACTT	GGTGGCTGAGTGGTCTAAAG	45.0	55.0	57.9	57.9	1531-1533 bp	1.0	84.3
moeB_p2	AAACACGCCTTGAAGGACTT	ACTGCCAAAAATCTGTCGGT	45.0	45.0	57.9	58.0	1896-1897 bp	0.666667	70.1
moeB_p3	CCTCGGCATTAGGTTTTGGA	ACTGCCAAAAATCTGTCGGT	50.0	45.0	57.9	58.0	1872-1873 bp	0.666667	69.5
moeB_p4	GAACCCTCGGCATTAGGTTT	ACTGCCAAAAATCTGTCGGT	50.0	45.0	57.9	58.0	1876-1877 bp	0.666667	69.5
moeB_p5	AGATGCTGGTGGCGTTTTTA	ACTGCCAAAAATCTGTCGGT	45.0	45.0	58.1	58.0	2260-2261 bp	0.666667	69.4